A product that comes to market looks very beautiful and fascinating. When we see it, we feel to take and use it without a second thought about its purity and sustainability. If we don’t think of it, then, is anyone else thinking about it? Yes, it’s the long way of tests it has passed and come to the customers. The test of antimicrobial activity method focuses and gives the specifications on standards. It checks the quality and essential standards of each product and sends it to the market.
There are different methods to analyze and check the antimicrobial activity of antimicrobial agents under several conditions. The ASTM e 2149 test method is used to determine how resistant specimens are treated with a non-leaching antimicrobial agent under dynamic contact conditions. It is sometimes known as the "shake flask test," a recommended method for evaluating antimicrobial additives.
About ASTM E2149
The concentrated antimicrobial agents are grown in liquid culture and allowed further. This test method can be used to examine a variety of treated substrates and a wide range of microorganisms. The diversity of measuring the effect of contamination due to hard water, proteins, blood, serum, various chemicals, and other pollutants is enabled by using treated substrates in this test method, which can be subjected to a wide variety of physical/chemical stresses or manipulations.
What can be tested?
The standard organism provided for the ASTM E2149 method is Escherichia coli ATCC 25922. Additional species and a jump from 1 hour to 24 hours of interaction time, or other periods, can be requested.
Strength of ASTM E2149
The ASTM E2149 method specifies the target beginning microbial concentration, making comparisons of different antimicrobial agents highly repeatable.
It is written so that it allows researchers to customize the procedure to test antimicrobial items of various forms and sizes.
It is a reproducible method of testing.
It maintains the accuracy and efficacy of results by their quality.
Weak of ASTM E2149
In more realistic scenarios (such as when a dilute suspension of bacteria is placed directly on a surface), the ASTM E2149 test does not create an appreciable antibacterial effect.
No clear criteria are set regarding the microbial activity and the product.
The contact time chosen for the study has a significant impact on its results.
As is already mentioned above, the ASTM E2149 method, titled "Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions," is a sensitive test that is frequently used to determine the antimicrobial activity of non-leaching, irregularly shaped, or hydrophobic surfaces. Simply said, when antimicrobial surfaces are shaken around in a microbially contaminated solution, it assesses their antimicrobial activity.
The results permit or allow quantifying the antimicrobial agent's efficacy on the treated surface by determining whether the reduction in bacteria is considerable compared to the inoculum or the untreated control. If the antimicrobial component leaches, the results are judged unreliable, and the antimicrobial must be neutralized when the product is retested.